Titlebook: RNA Detection; Methods and Protocol Imre Gaspar Book 2018 Springer Science+Business Media LLC 2018 Drosophila.ECHO-liveFISH.FISH.RCas9.FISS

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,Quantification of 2′-,-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Pr recently published accurate and sensitive detection of these modifications on native RNA at a single base resolution by high-throughput sequencing technologies. Relative quantification of these modifications is still under progress and would probably reduce the number of false positives due to 3D R

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Axon-TRAP-RiboTag: Affinity Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo, tagged ribosomal protein. We developed axon-TRAP-RiboTag, a TRAP-based method that allows purification and identification of translated mRNAs from distal neuronal axons in mouse, and identified more than 2000 of translated mRNAs in retinal ganglion cell (RGC) axons in vivo. The use of Cre-negative

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LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled thod utilizes off-the-shelf reagents and direct lysis of the cells without RNA purification, making it a simple and relatively cheap method with high reproducibility and sensitivity compared to previous methods. The advantage with LCM-seq is also that tissue sections are kept intact and thus the pos

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Single mRNA Molecule Detection in ,,s increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolu

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Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embrygmentation, transcripts can be assigned to different cellular compartments resulting in quantitative information on transcript levels at subcellular resolution. The use of smFISH in zebrafish has been limited by the lack of protocols and an automated image analysis pipeline for samples of multicellu