Titlebook: RNA Detection; Methods and Protocol Imre Gaspar Book 2018 Springer Science+Business Media LLC 2018 Drosophila.ECHO-liveFISH.FISH.RCas9.FISS

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Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH,iving cells. Using chemically engineered linear oligonucleotide probes that emit fluorescence upon hybridization to target RNA, we have devised an imaging method suitable for studies of the dynamic regulation of nuclear RNPs, an important and yet poorly understood cellular pathway of gene expression

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Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers, Here, we describe a novel live-cell RNA imaging method using fluorophore- and quencher-binding aptamers, which can be genetically fused to the ROI. In this method, new membrane permeable and nonfluorescent fluorophore–quencher conjugates were utilized, and we showed that their fluorescence increase