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Titlebook: RNA Biochemistry and Biotechnology; Jan Barciszewski,Brian F. C. Clark Book 1999 Springer Science+Business Media Dordrecht 1999 DNA.Elonga

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Metal Ion - Induced Cleavages in Probing of RNA Structure,n example, yeast tRNA., undergoes specific fragmentation in the D-loop in the presence of Pb. [1–3] and other ions: Eu.[4,5], Mn.[6] and Mg.[7,8]. It has been shown, based on X-ray analysis of yeast tRNA. crystals, that a Pb. ion induces the reaction acting from its tight metal ion-binding pocket [9
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Protein-DNA Recognition,processes. I will review the principles of DNA recognition that have emerged from the large number of high-resolution structures determined over the last 10 years. The DNA-binding domains of transcription factors exhibit surprisingly diverse protein architectures, yet all achieve a precise complemet
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Recognition of One tRNA by Two Classes of Aminoacyl-tRNA Synthetase,, the known lysyl-tRNA synthetases are subclass He-type aminoacyl-tRNA synthetases whereas some archaea and bacteria have been shown to contain an unrelated class I-type lysyl-tRNA synthetase. We have now examined substrate recognition by a bacterial (from .) and an archaeal (from .) class I lysyl-t
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,Aminoacylation of tRNA Induces a Conformational Switch on the 3’-Terminal Ribose,. The aromatic adenine-76 is usually located in a lipophilic binding pocket of the tRNA binding proteins. The ribofuranose ring of nucleosides is not planar and can adopt a C3’-.-C2’-. (north sugar, N) or C2’-.-C3’-. (south sugar, S) conformation. This sugar pucker defines the position of the nucleo
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Point Mutants of Elongation Factor Tu from , Impaired in Binding Aminoacyl-tRNA,ein engineering process is cyclic and runs through the following steps: design of substitution based on structural information, site-directed mutagenesis, expression and purification of mutant protein and finally a functional and structural characterisation..We have used the translational elongation
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RNA Structure and RNA-Protein Recognition During Regulation of Eukaryotic Gene Expression,e excision of non-coding regions (pre-mRNA splicing). During these maturation events, mRNAs are complexed in ribonucleoprotein particles with proteins that recognize specific RNA sequences to affect these different regulatory steps. The assembly of large ribonucleoproteins performing RNA splicing (s
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