Titlebook: RNA Biochemistry and Biotechnology; Jan Barciszewski,Brian F. C. Clark Book 1999 Springer Science+Business Media Dordrecht 1999 DNA.Elonga

深渊

RNA-Aptamers for Studying RNA Protein Interactions,, elongation factor G and release factor 1 from . which carry a terminal histidine-tag were immobilized on Ni.-agarose and used for selection of RNA-aptamers from a library of random RNA sequences. RNA molecules which bind to particular translation factors were characterized by UV-melting, chemical

和蔼

Probing of Ribonucleoprotein Complexes with Site-Specifically Derivatized RNAs,cess requires the development of a suitably simplified in vitro system. A system presented here is based on the use of two separate, trans-acting RNA molecules encompassing the two splice sites, which are together assembled into spliceosome complexes and undergo both steps of splicing. The small siz

Chandelier

The IRE Model for Families of RNA Structures,e regulation of ferritin mRNA by iron in animals, an older example of mRNA regulation, has been extended to other mRNAs of iron and oxidative metabolism. The regulatory element (IRE) is conserved in animal mRNAs encoding the transferrin receptor (TfR), m-aconitase, erythroid aminolevulinate synthase

遗传学

condone

Definitive

Recurrent RNA Motifs,ernai loops» for which high-resolution structures also exist, such as loop E of 5S rRNA and the sarcin/ricin loop of 23S rRNA. Such «dictionaries» of isosteric, context-specific pairings allow one to identify occurrences of the same motif in other molecules.

Focus-Words

RNA Structure and RNA-Protein Recognition During Regulation of Eukaryotic Gene Expression,ry protein factors. Understanding the molecular basis of RNA-protein recognition is therefore necessary to understand these regulatory events and to learn how to exogeneously regulate gene-expression.

删减

Metal Ion - Induced Cleavages in Probing of RNA Structure,,10]. This gave rise to an experimental approach that uses Pb. and other ions to localize some metal ion binding sites as well as to probe the structure of RNA molecules in the vicinity of the bound ions.

公式

Book 1999uction andcomputer modelling. Many papers describe the possibility of commercialapplication of RNA biotechnology. One article discusses the impact ofdirect democracy on basic science supporting biotechnology. ..Readership:. Advanced graduate students, Ph.D. students and youngscientists as well as specialists in the field.

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