Titlebook: RNA Biochemistry and Biotechnology; Jan Barciszewski,Brian F. C. Clark Book 1999 Springer Science+Business Media Dordrecht 1999 DNA.Elonga

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Point Mutants of Elongation Factor Tu from , Impaired in Binding Aminoacyl-tRNA, modification and cross-linking studies supplemented with protein engineering studies. Recently, the structure of the ternary complex, EF-Tu:GTP:aatRNA, was solved in our lab allowing a structural interpretation of our mutational studies. Our mutants impaired in binding aa-tRNA could be split into t

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RNA-Aptamers for Studying RNA Protein Interactions,mers might be useful inhibitors of reactions which depend on RNA protein interactions. The high specificity and high affinity of RNA-aptamers for proteins may also permit their use as reporter molecules. Fluorescence labeling of RNA-aptamers is required for such applications.

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Probing of Ribonucleoprotein Complexes with Site-Specifically Derivatized RNAs,d to derivatize the 5’SS RNA with heterobifunctional photo-crosslinking reagents and to probe the spliceosomal components located in proximity of the active site of the complex. Several crosslinked species can be detected under splicing conditions. These include UI and U6 snRNAs, and a number of pro

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The IRE Model for Families of RNA Structures,hile control of nuclease binding/activity appears to be the mechanism of IRE-dependent regulation for TfR and Nramp2 synthesis. A common secondary structure is shared among the isoIREs which includes a hairpin loop (CAGUGX) and a base paired stem with an interruption or “hinge” in mid-stem. Stem bas

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Functional Analysis of RNA Signals in the HIV-1 Genome by Forced Evolution,ncoded within the repeat region (R element) of the HIV-1 genome: the TAR and polyA hairpins. The different repair strategies observed for mutant forms of these hairpin structures will be described in detail, followed by a discussion of the functional insight gained by these studies. Because the R re

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Osamu Nureki,Shun-ichi Sekine,Atsushi Shimada,Takashi Nakama,Shuya Fukai,Dmitry G. Vassylyev,Ikuko S

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Andreas Schlosser,Bernd Blechschmidt,Barbara Nawrot,Mathias Sprinzl

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M. Sprinzl,H.-P. Hoffmann,S. Brock,M. Nanninga,V. Hornungult questions are marked by a diamond and can be given to advanced readers. Paragraphs marked by /// are very important for the understanding of the studied material and together they can serve a brief summary 978-94-007-3815-7978-94-007-2205-7Series ISSN 1437-0387 Series E-ISSN 2197-6643