Titlebook: Quantitative Real-Time PCR; Methods and Protocol Roberto Biassoni,Alessandro Raso Book 2014 Springer Science+Business Media New York 2014 D

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Posttranscriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA e real mRNA and miRNA transcriptomic data as well as in silico target predictions. This chapter addresses the workflow and methods one can apply for expression profiling and the integrative analysis of mRNA and miRNA data, as well as how to analyze and interpret results, and how to build up models of posttranscriptional regulatory networks.

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Book 2014bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications

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Twenty Years of qPCR: A Mature Technology?,ts use has been grown exponentially. More recent technological advancements have extended the field of applications ranging from high-resolution melting detection to digital PCR. Nowadays, it is a very accessible technique, but some pitfalls should be overcome in order to achieve robust and reliable

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Introduction to Digital PCR,a routine molecular biology assay. This exciting new technique provides quantitative and detection capabilities that by far surpass other methods currently used. This chapter is an overview of some of the applications currently being performed using dPCR as well as the fundamental concepts and techn

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mRNA and microRNA Purity and Integrity: The Key to Success in Expression Profiling,s, using micro-array, RT-qPCR (Reverse-Transcription quantitative PCR), or Next-Generation-Sequencing by RNA-Seq or small-RNA Seq. Therefore, assessment of RNA integrity and purity is very essential prior to gene expression analysis of sample RNA to ensure the accuracy of any downstream applications

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A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detect of neoplastic progression. HR-HPV detection and genotyping is the most effective and accurate approach in screening of the early cervical lesions and cervical cancer, although also the HR-HPV DNA load is considered an ancillary marker for persistent HPV infection..Here, it is described an in-house