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Titlebook: Quantitative Real-Time PCR; Methods and Protocol Roberto Biassoni,Alessandro Raso Book 2014 Springer Science+Business Media New York 2014 D

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发表于 2025-3-21 16:37:37 | 显示全部楼层 |阅读模式
书目名称Quantitative Real-Time PCR
副标题Methods and Protocol
编辑Roberto Biassoni,Alessandro Raso
视频video
概述Includes cutting-edge methods and protocols in qPCR techniques.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes su
丛书名称Methods in Molecular Biology
图书封面Titlebook: Quantitative Real-Time PCR; Methods and Protocol Roberto Biassoni,Alessandro Raso Book 2014 Springer Science+Business Media New York 2014 D
描述.Quantitative Real-Time PCR: Methods and Protocols. focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the .Methods in Molecular Biology. series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and authoritative, .Quantitative Real-Time PCR: Methods and Protocols. aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of inv
出版日期Book 2014
关键词Digital PCR; Microbiological detections; Nucleic acid measurement; Pathological applications; Quality is
版次1
doihttps://doi.org/10.1007/978-1-4939-0733-5
isbn_softcover978-1-4939-4665-5
isbn_ebook978-1-4939-0733-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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发表于 2025-3-21 21:49:03 | 显示全部楼层
Selection of Reliable Reference Genes for RT-qPCR Analysis,nce gene quality in subsequent studies. For screening studies including a large, unbiased set of genes (e.g., complete miRNome) an alternative normalization method can be used: global mean normalization. This chapter also describes how the data from such studies can be used to identify reference gen
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Clinical Applications Using Digital PCR,ib. These agents are effective at reducing out-of-control cell cycling and tumor proliferation, but only if downstream signaling kinases and phosphatases are not mutated. Known oncogenes such as BRAF V600E and KRAS G12/13 that are constitutively activated render these treatments ineffective. The use
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Book 2014y materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and authoritative, .Quantitative Real-Time PCR: Methods and Protocols. aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of inv
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Eddi Di Marco in cognitive science..This book conducts a sustained and detailed examination of the structure of tractable and intelligible reasoning in cognitive science and artificial intelligence. Working from the perspec978-3-030-36235-5978-3-030-36233-1Series ISSN 0166-6991 Series E-ISSN 2542-8292
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