Titlebook: Quantitative Real-Time PCR; Methods and Protocol Roberto Biassoni,Alessandro Raso Book 2014 Springer Science+Business Media New York 2014 D

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Real-Time PCR Detection of , in the Diagnosis of Community-Acquired Pneumonia,ic acid can be amplified by PCR upon clinical sample extraction using specific primers for classical qualitative PCR and primers and probes for real-time PCR..Here we describe the Scorpion-probe real-time PCR-based assay that offers thermodynamic advantages due to its kinetic reaction and provides f

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A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition,ue cultures is a major problem because it can determine various and severe alterations in cellular function..Thus methods able to detect and trace cell cultures with Mollicutes contamination are needed in the monitoring of cells grown under good manufacturing practice conditions, and cell lines in c

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A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR,umor. Up-to-date conclusive diagnosis is generally achieved through fluorescence in situ hybridization or karyotyping analysis..Herein, we report an alternative, reliable assay for rapidly defining vertical cancer transmission to the fetus by using quantitative polymerase chain reaction. Our assay i

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Posttranscriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA miRNA) regulatory interactions facilitate the understanding of fine-tuning of gene expression and might allow better estimation of protein synthesis. However, in silico predictions of mRNA–microRNA interactions do not take into account the specific transcriptomic status of the biological system and

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Clinical Applications Using Digital PCR, (Strain and Richman, Curr Opin HIV AIDS 8:106–110, 2013) and becoming prevalent in the nonscientific literature. Instead of generically using a “one treatment fits all” approach that may have varying levels of effectiveness to different patients, patient-specific molecular profiling based on the ge

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Developing Noninvasive Diagnosis for Single-Gene Disorders: The Role of Digital PCR, material for noninvasive prenatal diagnosis (NIPD) from early pregnancy. The relatively high levels of maternal background can make detection of paternally inherited point mutations challenging. Diagnosis of inheritance of autosomal recessive disorders using qPCR is even more challenging due to the

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1064-3745 notes and implementation advice from the experts.Includes su.Quantitative Real-Time PCR: Methods and Protocols. focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which r

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