Titlebook: Non-Viral Gene Delivery Vectors; Methods and Protocol Gabriele Candiani Book 2016 Springer Science+Business Media New York 2016 conventiona

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Polyallylamine Derivatives: Novel NonToxic Transfection Agentsf polyplexes using dynamic light scattering, ζ-potential measurements, gel retardation assay, and atomic force microscopy. Moreover, we provide protocols for the transfection of HeLa cell line by polyplexes, determination of their cytotoxicity, cell uptake, and intracellular trafficking.

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1064-3745 ation advice from the experts.Includes supplementary materiaThis volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I i

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Book 2016 delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II cov

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https://doi.org/10.1007/978-1-4939-3718-9conventional bolus gene delivery vectors; cell culture medium; stimuli-responsive bolus transfectants;

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Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells corrective gene therapy for the treatment of neoplasms and other disorders of a genetic origin. Here, a step-wise account of the assembly and characterization of hepatocellular carcinoma cell-specific DNA lipoplexes and their cytotoxicity assessment in and delivery to the human hepatocellular carcinoma cell line HepG2 is given.

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Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA Deliverysed on targeted anionic lipid-coated polyplexs for therapeutic delivery of microRNA (miRNA) mimics. These LNPs are particularly suited for therapeutic delivery of oligonucleotide agents to leukemia cells.