| 书目名称 | Non-Viral Gene Delivery Vectors |
| 副标题 | Methods and Protocol |
| 编辑 | Gabriele Candiani |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery—also termed reversetransfection—and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful .Methods in Molecular Biology. series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls..Cutting-edge a |
| 出版日期 | Book 2016 |
| 关键词 | conventional bolus gene delivery vectors; cell culture medium; stimuli-responsive bolus transfectants; |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-4939-3718-9 |
| isbn_softcover | 978-1-4939-8118-2 |
| isbn_ebook | 978-1-4939-3718-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2016 |
发表于 2025-3-21 17:43:25
