Titlebook: Non-Viral Gene Delivery Vectors; Methods and Protocol Gabriele Candiani Book 2016 Springer Science+Business Media New York 2016 conventiona

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Margarida I. Simão Carlos,Andreas Schätzlein,Ijeoma Uchegbu

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Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micellesng interaction with nucleic acid. Herein the main issues related to complex formation and in vitro administration are illustrated with key examples, such as liposome-DNA plasmid (pDNA) association and micelles-siRNA complexes.

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Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposome protocols for the elaboration of niosomes and nioplexes and their physicochemical characterization that guarantees the quality criteria of the formulation in terms of size, morphology, ζ-potential, and stability.

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Polymer Based Gene Silencing: In Vitro Delivery of SiRNA). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a polymer are presented in this chapter using EAGC as an example. The steps include: the transfection of cells with EAGC-siRNA polyplexes and protein detection by a Western Blotting assay.

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Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA an AL, a zwitterionic lipid (ZL), and a plasmid DNA (pDNA), their electrostatic interaction being necessarily mediated by divalent cations, such as Ca.. In the present chapter, we summarize the protocols optimized for the mentioned characterization techniques.