Titlebook: Neural Reprogramming; Methods and Protocol Henrik Ahlenius Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Som

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Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes ited availability of human primary astrocytes for cell culture studies hinders our understanding of their physiology and precise role in disease development and progression. Here, we describe a detailed step-by-step protocol to rapidly and efficiently generate functionally mature induced astrocytes

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Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generh the conductive myelin formation and the energy support via lactate can be affected in diseases, such as multiple sclerosis and amyotrophic lateral sclerosis, respectively. Therefore, human disease modeling is needed to gain more mechanistic insights to drive drug discovery research. Here, patient-

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Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neurondidates for treatment of neurodegeneration. Acute studies in human brain slices are limited by the lifetime of the tissue and focus mainly on hippocampus slice preparation. Here we describe the derivation of human organotypic slice cultures of cortical origin, which can be kept in culture for up to

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