Titlebook: Neural Reprogramming; Methods and Protocol Henrik Ahlenius Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Som

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Isaac Canals,Henrik AhleniusIt is being discovered that there is a non-negligible class of nonlinear field equations which, if not bearing exact soliton solutions, clearly have both spatially extended and spatially compact solutions, that these solutions are functionally independent to a high degree (if not exactly so), and th

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Patrimony

Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons,ategy is based on the co-expression of Bcl-2 and the transcription factor Neurog2 that produces mostly excitatory neurons. However, the method can be easily adapted to achieve alternative neuronal subtypes by using additional transcription factors, such as Isl1 for motor neurons. Therefore, our appr

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Incorruptible

Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes and support of synapse formation and function, making them suitable models for studying the role of astrocytes in health and disease. Moreover, we describe a procedure for cryopreservation of iAs for long-term storage or shipping. Finally, we provide the required information needed to set up cocultu

reflection

Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generigodendrocytes in just 20–24 days. After a neural induction phase of 8–12 days, SOX10 is overexpressed either with the use of lentiviral vectors or via engineered iPSCs, which inducibly overexpress SOX10 after doxycycline addition. Using this last method, a pure O4. cell population is achieved after

勾引

Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo,ermits functional assessment using patch-clamp electrophysiology. For in vitro reprogramming we further provide an optimized coating condition that allows a long-term maturation of human induced neurons in vitro.

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Generosity

1064-3745 mental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research..978-1-0716-1603-1978-1-0716-1601-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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ers, and their students with the proper methods and tools to solve the soliton equations, and to discover the new possibilities of using solitons in multi-disciplinary areas ranging from telecommunicati978-1-0716-2457-9Series ISSN 2629-2327 Series E-ISSN 2629-2343