Titlebook: Neural Reprogramming; Methods and Protocol Henrik Ahlenius Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Som

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Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neurompus slice preparation. Here we describe the derivation of human organotypic slice cultures of cortical origin, which can be kept in culture for up to 6 weeks. This method enabled us to test the system in coculture with reprogrammed neurons and show its feasibility in neuronal cell integration experiments in human-to-human grafting situation.

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Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs),pidly derive functionally mature neurons directly from human pluripotent stem cells (hPSCs) through a brief induction of defined transcription factors. In this chapter, we describe the detailed methods used to attain the direct conversion from hPSCs to glutamatergic and GABAergic iN cells.

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Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions,contribution (activation or repression) of specific neuronal reprogramming factors during direct cell fate conversion. This method can be extended to every reprogramming regime to enable the functional categorization of any transcription factor.

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1064-3745 expertsThis detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as w

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Transcriptional Profiling During Neural Conversion,such, transcriptional profiling is a key component toward understanding this process. In this chapter, we will discuss methods of elucidating transcriptional networks during neuronal reprogramming and considerations that should be incorporated in experimental design.

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