Titlebook: Nanotechnology for Nucleic Acid Delivery; Methods and Protocol Manfred Ogris,Haider Sami Book 2019Latest edition Springer Science+Business

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Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection,d synthesis. However, the trifluoroacetic acid (TFA)-mediated removal of acid-labile protecting groups and cleavage from the resin can be accompanied by side products caused by an addition of TFA to the double bonds of unsaturated fatty acids. These TFA adducts are converted into hydroxylated deriva

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Synthesis of Bioreducible Polycations with Controlled Topologies, stability in extracellular physiological condition and bioreduction-triggered release of the genetic material. Further benefits of bioreducible polycations include decreased cytotoxicity due to intracellular reducing environment in the cytoplasm that contains high levels of reducing molecules such

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Histidine-Rich Cationic Cell-Penetrating Peptides for Plasmid DNA and siRNA Delivery,rfering RNA (siRNA) into mammalian cells. Their ability to bind and protect nucleic acids and then disrupt membranes when activated at low pH enables them to harness the endocytic machinery to deliver cargo efficiently and with low associated toxicity. This chapter describes protocols for the chemic

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Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugration of oligonucleotide conjugates depends critically on the chemical nature of the used ligand and linker, there is no general and universal procedure. Here, we present a detailed, quick, and facile protocol for attaching fluorescent dyes or cross-linkers of variable chemical stability to oligonu

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Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery,ained interest as stabilized monodisperse systems showing potential for the delivery of hydrophobic drugs as well as of larger biomolecules such as nucleic acids. Introduction of pH-sensitive histidine groups at the surface of the micellar PDA systems allows for efficient delivery of siRNA resulting

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