Titlebook: Nanotechnology for Nucleic Acid Delivery; Methods and Protocol Manfred Ogris,Haider Sami Book 2019Latest edition Springer Science+Business

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,Preparation, Characterization, and In Vitro Evaluation of Lipidoid–Polymer Hybrid Nanoparticles for aqueous buffer is emulsified by sonication with an organic phase containing lipid and polymer into a primary emulsion. Subsequently, the primary emulsion is emulsified with a secondary water phase containing polyvinyl alcohol by sonication, and the organic phase is evaporated, eventually resulting

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Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles,ffolds (i.e., hydrogel-mediated delivery) to efficiently deliver genes. This chapter includes protocols for surface-mediated DNA delivery focusing on the simplest and most effective methods, which include nonspecific immobilization of DNA complexes (both polymer and lipid vectors) onto serum-coated

anaerobic

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Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery,netic responsiveness and ultrasound contrast effects. Protocols are given for the transfection procedure in adherent cells, evaluation of the association of the magnetic vectors with the cells, reporter gene expression analysis, and cell viability assessment.

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Agility

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