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Titlebook: Nanotechnology for Nucleic Acid Delivery; Methods and Protocol Manfred Ogris,Haider Sami Book 2019Latest edition Springer Science+Business

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发表于 2025-3-21 16:05:40 | 显示全部楼层 |阅读模式
书目名称Nanotechnology for Nucleic Acid Delivery
副标题Methods and Protocol
编辑Manfred Ogris,Haider Sami
视频video
概述Includes cutting-edge techniques.Provides step-by-step details essential for reproducible results.Contains key advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Nanotechnology for Nucleic Acid Delivery; Methods and Protocol Manfred Ogris,Haider Sami Book 2019Latest edition Springer Science+Business
描述This detailed second edition volume expands upon the prior edition by addressing newly emerged technologies as well as improved existing protocols in nucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealing with application and imaging of such carrier systems in vivo. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, Second Edition. serves as an ideal guide to researchers seeking to use this vital area of study in their own lab work or to progress knowledge within the field itself..
出版日期Book 2019Latest edition
关键词Delivery systems; Toxicology; Bioconjugates; Nanocarriers; Nanoformulations; Nucleic acid therapies; Bioco
版次2
doihttps://doi.org/10.1007/978-1-4939-9092-4
isbn_ebook978-1-4939-9092-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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发表于 2025-3-21 23:17:06 | 显示全部楼层
Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery,icroscopy, and diffusion ordered spectroscopy. Moreover, we describe standardized biological tests to evaluate the silencing efficiency by the use of a cell line constitutively expressing the luciferase reporter gene.
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Layer-by-Layer Assembled Nanoparticles for siRNA Delivery,e the manufacturing of poly(lactic-.-glycolic acid) NPs coated with siRNA for gene silencing. Positively charged polyethyleneimine and negatively charged nucleic acids form the polyelectrolyte shell. Finally, the NPs are functionalized with hyaluronic acid, a polysaccharide which targets the CD44 receptor.
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Book 2019Latest editionnucleic acid delivery via nanotechnology. The book addresses topics ranging from chemical synthesis of macromolecules and bioconjugates, novel and established nanoformulations, characterization of these nanoformulations for biophysical, biological and toxicological aspects, and also protocols dealin
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Synthesis of Bioreducible Polycations with Controlled Topologies,ations include decreased cytotoxicity due to intracellular reducing environment in the cytoplasm that contains high levels of reducing molecules such as glutathione. Here, we describe the synthesis of bioreducible polycations with emphasis on methods to control their topology.
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