Titlebook: Nanoscale Imaging of Synapses; New Concepts and Opp U. Valentin Nägerl,Antoine Triller Book 2014 Springer Science+Business Media New York 2

mercenary

https://doi.org/10.1007/978-1-4614-9179-8live-cell fluorescence microsopy; nanoscopy; neurons and glia; physiology; synapses

傻瓜

Nanoscale Imaging of Protein Molecules at the Postsynaptic Density,ld EM coupled with tomography identifies a class of membrane-associated vertical filaments in the PSD core as PSD-95, the major scaffolding protein. Acute knockdown of PSD-95 with RNAi leads to loss of patches of electron-dense PSD material, including loss of vertical filaments and their associated

饥荒

glans-penis

Live-Cell PALM of Intracellular Proteins in Neurons,vantages over other imaging modalities. First, it permits the localization of single molecules with precision on the order of tens of nanometers and the rendering of neuronal structures at high resolution. As this technique utilizes expressed proteins tagged with photoconvertible fluorescent protein

forager

esculent

delusion

Imaging-Based Measures of Synaptic Tenacity,ar dynamics of synaptic components and on quantitative measures of synaptic tenacity—the capacity of synapses to maintain their characteristics over time. These include fluorescence recovery after photobleaching (FRAP), fluorescence recovery after photoactivation (FRAPA), and several analytical tool

MORPH

Zooming in on the (Peri)synaptic Extracellular Matrix,artite system including the pre- and postsynapse, associated astroglial terminals, and the ECM, in which there is an exchange of signals between all four components. This review is to give a short introduction to neural ECM; to summarize available electron microscopy data on expression of perisynapt

胆小鬼

Two-Photon Excitation STED Microscopy for Imaging Synapses and Glia in Acute Brain Slices,with a powerful array of complementary techniques, such as photo-uncaging, fluorescence recovery after photobleaching (FRAP), and patch-clamp electrophysiology, giving experimental access to a rich set of molecular, structural, and functional data..Here, we present a primer on two-photon excitation

LIMN

0893-2336 he various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.978-1-4939-4918-2978-1-4614-9179-8Series ISSN 0893-2336 Series E-ISSN 1940-6045