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Titlebook: Mechanobiology; Methods and Protocol Ronen Zaidel-Bar Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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书目名称Mechanobiology
副标题Methods and Protocol
编辑Ronen Zaidel-Bar
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Mechanobiology; Methods and Protocol Ronen Zaidel-Bar Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to
描述This detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features methods to quantify the mechanical properties of cells and adhesion proteins, to expose cells to external mechanical forces, to quantitatively characterize mechano-responses at various scales, to measure forces applied by cells on the extracellular matrix, as well as chapters on force measurement inside cells, probing cell signaling and gene expression in response to force, and biophysical modeling of cell shape and protein dynamics. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Mechanobiology: Methods and Protocols. aims to provide meaningful tools for cell and developmental biologists approaching the study of cell and tissue dynam
出版日期Book 2023
关键词Mechanotransduction; Gene expression; Mechanosensing; Cellular forces; Mechanical cues; Biophysical model
版次1
doihttps://doi.org/10.1007/978-1-0716-2851-5
isbn_softcover978-1-0716-2853-9
isbn_ebook978-1-0716-2851-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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书目名称Mechanobiology影响因子(影响力)学科排名




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Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using e energetic cost of increasing the surface area of the plasma membrane, has been shown to regulate a plethora of cellular processes, ranging from leading edge formation to phagocytosis and membrane trafficking. Here, we describe the measurement of this key mechanical property of the cell surface usi
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Photoresponsive Hydrogels for Studying Mechanotransduction of Cellsd by a spatial gradient of stiffness of the underlying substrate, requires materials such as polyacrylamide, polyethylene glycol, or PDMS, in which the stiffness can be controlled in a spatiotemporal manner. Here, we describe the synthesis of an .-nitrobenzyl-based photocleavable cross-linker and it
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