Titlebook: Mechanobiology; Methods and Protocol Ronen Zaidel-Bar Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretchinge fabrication of defined elastic substrates and their application in cyclic stretching of cultured cells in a custom-made cell stretcher device. We focus on the detailed description of the system and provide a possibility for mechanoresponse characterization, using the analysis of actin stress fiber

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Book 2023gents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Mechanobiology: Methods and Protocols. aims to provide meaningful tools for cell and developmental biologists approaching the study of cell and tissue dynam

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Kenji Nishizawa,Claire Chardès,Raphaël Clément,Pierre-François Lenne

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Book 2023 single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features methods to quantify the mechanical properties of cells and adhesion proteins, to expose cells to external mechanical forces, to quantitatively characterize mec

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In Situ Measurements of Cell Mechanical Properties Using Force Spectroscopyl properties of single cells and even at the subcellular level. Particularly, nanoindentation measurements using atomic force microscopy (AFM) mechanically probes single cells and even allows mapping of these traits. This chapter discusses these measurements from the experimental design to the analysis.

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Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using us membrane tension components, such as apparent membrane tension and membrane-to-cortex attachment (MCA). We discuss the biophysical background (.), required materials (.), sample preparation (.), AFM-probe calibration and functionalization (.), force curve acquisition (.) and data analysis and representation (.).