Titlebook: Mechanobiology; Methods and Protocol Ronen Zaidel-Bar Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Quantifying Strain-Sensing Protein Recruitment During Stress Fiber Repairtrain- or force-sensing and trigger downstream signaling mechanisms that can facilitate repair at these strain sites. Here we describe a method using high-resolution microscopy to screen and quantify the mechanosensitive recruitment of proteins to these stress fiber strain sites. Strain sites are in

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Quantification of Invadopodia Formation and Matrix Degradation Activityastases in distant organs. This complex and multistep process remains one of the major causes of mortality in cancer patients worldwide. Multiple studies have highlighted the role of actin-rich structures called invadopodia (“invasive feet”), which adhere to the matrix, contain and secrete matrix-de

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Measuring Cellular Traction Forces with Micropillar Arraysments on the piconewton scale. Here we describe a specialized method that employs elastic polydimethylsiloxane (PDMS) micropillar arrays, which cells can adhere to and bend. The flexibility of the pillars correlates with their heights; the longer they are, the easier they are to bend. Thus, an array

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Mechanobiology978-1-0716-2851-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the , Embryongle cell-cell junctions in a developing tissue. We further extended the approach to two-point laser manipulation to enable extensive remodeling of cell-cell junctions. Here, we describe two-point laser manipulation and its implementation to probe the mechanics of cell junctions in the . embryo.