Titlebook: Mammary Stem Cells; Methods and Protocol Maria del Mar Vivanco Book 2015 Springer Science+Business Media New York 2015 breast cancer.embryo

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Shotgun Proteomics on Tissue Specimens Extracted with Acid Guanidinium-Thiocyanate-Phenol-Chlorofor method is widely used for RNA extraction for gene expression analysis. However, due to difficulties in redissolving pelleted proteins from the organic phase, protein analysis has only been limitedly reported. Current shotgun mass spectrometry-based methods, however, require minute amounts of sample

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Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments,or determining the relative abundance of proteins in complex mixtures, like plasma or whole tissue. This technique combines 1,000-fold enrichment potential of antibodies for target peptides with the selectivity of multiple reaction monitoring mass spectrometry (MRM-MS). Using heavy isotope-labeled p

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Lentiviral Transduction of Mammary Epithelial Cells, integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100 % of the cells in a culture and possible to infect cells simultaneously with multiple vectors, greatly facilitating studies on malignant transformation. We present simple protocols to produce and t

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The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads,ment. This technique involves the grafting of tissue fragments or isolated cells into the mammary fat pads of prepubertal mice from which the endogenous epithelium has been surgically removed. Such transplantation assays are particularly useful for the analysis of morphogenetic potential and stem ce

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Humanization of the Mouse Mammary Gland,tween human and mice limit full understanding of this fundamental process. Humanization of the mouse mammary gland by injecting immortalized human breast stromal cells into the cleared murine mammary fat pad enables the growth and development of human mammary epithelial cells or tissue. This facilit

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Modeling the Breast Cancer Bone Metastatic Niche in Complex Three-Dimensional Cocultures,in the last years. Metastatic breast cancer remains incurable. There is compelling evidence that dissemination of breast cancer cells at distant sites is an early event. At the time of detection and diagnosis, patients have disseminated breast cancer cells in the bone marrow. Only in half of these p

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Mammary Cancer Stem Cells Reinitiation Assessment at the Metastatic Niche: The Lung and Bone,. Building on this observation, it has also been suggested that MCSCs are responsible for metastasis as well as evasion and resistance to therapeutic treatments. MCSC reinitiating potential is usually tested by implantation of limited amounts of cells orthotopically or subcutaneously, yet this poorl

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