书目名称 | Mammary Stem Cells |
副标题 | Methods and Protocol |
编辑 | Maria del Mar Vivanco |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and .in vivo. lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful .Methods in Molecular Biology .series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory..Authoritative a |
出版日期 | Book 2015 |
关键词 | breast cancer; embryogenesis; fluorescent activated cell sorting; gene expression; in vivo lineage traci |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-2519-3 |
isbn_softcover | 978-1-4939-4849-9 |
isbn_ebook | 978-1-4939-2519-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2015 |