Titlebook: Mammary Stem Cells; Methods and Protocol Maria del Mar Vivanco Book 2015 Springer Science+Business Media New York 2015 breast cancer.embryo

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Book 2015treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammar

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FACS Sorting Mammary Stem Cells,tated the identification of stem cells from the healthy mammary gland and from breast tumors. In this chapter we describe the protocol to use FACS to separate breast cancer stem cells, but most of the general principles discussed could be applied to sort other types of cells.

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Lentiviral Transduction of Mammary Epithelial Cells,ect cells simultaneously with multiple vectors, greatly facilitating studies on malignant transformation. We present simple protocols to produce and titrate lentiviral vectors, infect mammary epithelial cells, and check for contamination with replication-competent viruses.

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Mathematical Modelling as a Tool to Understand Cell Self-renewal and Differentiation,acter of transitions between differentiation stages. Advantages and constraints of the mathematical approaches are presented on examples of models of blood systems and compared to patients data on healthy hematopoiesis.

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The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads,ll activity in normal mammary epithelium and breast tumors. We describe here the main steps in the transplantation of epithelial fragments and isolated cells from mouse mammary glands and the various approaches currently used to evaluate the regeneration and self-renewal properties of mammary stem cells.

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