Titlebook: Mammalian Cell Engineering; Methods and Protocol Ryosuke Kojima Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 202

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Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells of mutation outcomes and frequencies induced by hundreds to thousands of different genome-editing reagents in mammalian cells. We have successful experiences of developing several key genome-editing tools using this protocol. The protocol also is designed to be compatible with robotic liquid handling systems for further scalability.

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Book 2021or future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two lo

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Construction of Caffeine-Inducible Gene Switches in Mammalian Cellsto implement caffeine-controlled gene expression systems into mammalian cells. Using an array of different implementation strategies, from reconstituting transcription factors to activating endogenous signaling pathways, allows for a wide range of sensitivity and capacity of the resulting caffeine-responsive gene switches.

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1064-3745 ation advice from the experts.This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book

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Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cellsingle-pass transmembrane receptors to activate endogenous signaling pathways. As of now, GEMS have been used for sensing prostate specific antigen (PSA), the synthetic azo dye RR120, caffeine, nicotine, rapamycin, the SunTag peptide, and a de novo designed protein displaying two viral epitopes. The