| 书目名称 | Mammalian Cell Engineering |
| 副标题 | Methods and Protocol |
| 编辑 | Ryosuke Kojima |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts. |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooti |
| 出版日期 | Book 2021 |
| 关键词 | dCas9-based chimeric receptors; SynNotch; SUPRA-CAR; Riboswitches; CRISPR |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-0716-1441-9 |
| isbn_softcover | 978-1-0716-1443-3 |
| isbn_ebook | 978-1-0716-1441-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
发表于 2025-3-21 18:26:46
