Titlebook: Leukemia Stem Cells; Methods and Protocol César Cobaleda,Isidro Sánchez-García Book 2021 Springer Science+Business Media, LLC, part of Spri

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书目名称Leukemia Stem Cells影响因子(影响力)




书目名称Leukemia Stem Cells影响因子(影响力)学科排名




书目名称Leukemia Stem Cells网络公开度




书目名称Leukemia Stem Cells网络公开度学科排名




书目名称Leukemia Stem Cells被引频次




书目名称Leukemia Stem Cells被引频次学科排名




书目名称Leukemia Stem Cells年度引用




书目名称Leukemia Stem Cells年度引用学科排名




书目名称Leukemia Stem Cells读者反馈




书目名称Leukemia Stem Cells读者反馈学科排名

歌唱队

Book 2021eagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and comprehensive, .Leukemia Stem Cells: Methods and Protocols. serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research..

oncologist

使入迷

Introduction and Classification of Leukemiasess in how we describe the nature of the “target” cells that genetic insults transform to give rise to the hematological malignancies. Here I describe how both longstanding and new information has influenced classifying, for diagnosis, the hematological malignancies.

Occupation

PCR Technology to Identify Minimal Residual Diseaseedure which has been developed and standardized (with minor modification in single labs) through the last 20 years of activity of the EuroMRD Consortium, a volunteer activity of expert laboratories that is continuously providing education, standardization, quality control rounds, and guidelines for interpretation of RQ-PCR data.

adjacent

Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignanciesregulation. So far, only one standardized academic protocol for this task has been published, developed, and validated within the EuroClonality-NGS working group. In this chapter we describe the materials and methods for amplicon library preparation for sequencing on Illumina MiSeq, and the bioinformatic pipeline for this protocol.

休息

Leukemic Stem Cell Culture in Cytokine-Free Mediumant after culture. This impedes development of new therapies for AML that target LSCs. Here, we present a simple strategy to culture LSCs in cytokine-free medium and to perform flow cytometric analysis of the resulting cell population for the characterization of LSCs maintenance and differentiation.

大暴雨

Book 2021and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the high

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