Titlebook: Leukemia Stem Cells; Methods and Protocol César Cobaleda,Isidro Sánchez-García Book 2021 Springer Science+Business Media, LLC, part of Spri

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978-1-0716-0812-8Springer Science+Business Media, LLC, part of Springer Nature 2021

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Leukemia Stem Cells978-1-0716-0810-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Analysis and Isolation of Mouse Leukemic Stem CellsHSCs) and leukemic stem cells (LSCs) can be highly purified by flow cytometry. Isolated HSCs and LSCs can be functionally identified by transplantation assays and can also be studied at the molecular level. Here we describe the flow cytometry methods for analysis and isolation of mouse HSCs and LSCs.

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Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)ukemic cells in the bone marrow. Most commonly, reverse transcriptase PCR is used to detect these translocations on RNA level. This technique has severe drawbacks, including sensitivity to contamination and instability of RNA. Here, we describe the . (GIPFEL) that overcomes these pitfalls.

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Isolation, Culture, and Manipulation of Human Cord Blood ProgenitorsThese characteristics, among others, make CB a good source of HSPCs not only for transplantation, but also for biomedical research purposes. Here we describe the methods for human CB-HSPCs isolation, as well as their culture and cryopreservation, viral transduction and sorting, and in vivo and in vitro assays in order to study leukemic processes.

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https://doi.org/10.1007/978-1-0716-0810-4Cancer stem cells; Hematopoietic stem cells; Tissue culture; LSCs; Human leukemias; Animal models

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Geoffrey Brownle or everyday language, common situations, requiring the use of arithmetic, geometry, or logic, for its solution. In dialogues, a procedure was developed to make decisions, based on answers and a record (knowledge base) of the occurrences of omission, while maintaining the communication process. Th

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Isidro Sánchez-García,César Cobaleda results show that 45% of users remain with the same provider for more than 12 months. It is concluded that the virtual assistant strategy reaches an acceptance rate of 71% and generates the beginning of customer loyalty for after-sales services in fixed Internet companies.