Titlebook: Leukemia Stem Cells; Methods and Protocol César Cobaleda,Isidro Sánchez-García Book 2021 Springer Science+Business Media, LLC, part of Spri

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Experimental Competitive Bone Marrow Transplant Assaysrrow cells from a healthy donor to the patient is an efficient solution to restore normal hematopoiesis. Bone marrow transplant in a mouse model is often used to study HSC function and capacity to repopulate an irradiated recipient. This protocol details the different steps of a competitive bone mar

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Leukemic Stem Cell Culture in Cytokine-Free Medium are believed to be a major cause of relapse in acute myeloid leukemia (AML). Despite the aggressive characteristics of acute leukemia, AML blasts are difficult to culture once removed from the patient, and LSCs, which are a minor fraction of the blast population, are especially difficult to transpl

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Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acidtransplantation but lack a matched donor. However, the limited number of HSCs within each UCB unit remains a major challenge for their use in regenerative medicine and HSC transplantation in adults. Efficient expansion of human HSCs in ex vivo cultures initiated with CD34. cells isolated from UCBs c

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Isolation, Culture, and Manipulation of Human Cord Blood ProgenitorsThese characteristics, among others, make CB a good source of HSPCs not only for transplantation, but also for biomedical research purposes. Here we describe the methods for human CB-HSPCs isolation, as well as their culture and cryopreservation, viral transduction and sorting, and in vivo and in vi

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César Cobaleda,Isidro Sánchez-GarcíaIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/585300.jpg