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Titlebook: Kidney Development; Methods and Protocol Odyssé Michos Book 2012 Springer Science+Business Media, LLC 2012 branching morphogenesis.cell mig

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书目名称Kidney Development
副标题Methods and Protocol
编辑Odyssé Michos
视频video
概述Focuses on mouse models but features other popular models as well.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes
丛书名称Methods in Molecular Biology
图书封面Titlebook: Kidney Development; Methods and Protocol Odyssé Michos Book 2012 Springer Science+Business Media, LLC 2012 branching morphogenesis.cell mig
描述.Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds.  One fundamental aspect of development biology is the concept of morphogenesis: understanding how a group of multipotent cells organize and differentiate into a complex organ.  In. Kidney Development: Methods and Protocols., expert researchers in the field detail different approaches to tackle kidney development. These approaches include culture and live imaging aspects of kidney development, analyzing the 3-dimensional aspects of branching morphogenesis as well as nephrogenesis, manipulation of the gene/protein expression during kidney development as well as in the adult kidney, and how to assess kidney malformation and disease. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical,. Kidney Developmen
出版日期Book 2012
关键词branching morphogenesis; cell migration; cell signaling; disease; ex vivo culture; kidney development; kid
版次1
doihttps://doi.org/10.1007/978-1-61779-851-1
isbn_softcover978-1-4939-6255-6
isbn_ebook978-1-61779-851-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2012
The information of publication is updating

书目名称Kidney Development影响因子(影响力)




书目名称Kidney Development影响因子(影响力)学科排名




书目名称Kidney Development网络公开度




书目名称Kidney Development网络公开度学科排名




书目名称Kidney Development被引频次




书目名称Kidney Development被引频次学科排名




书目名称Kidney Development年度引用




书目名称Kidney Development年度引用学科排名




书目名称Kidney Development读者反馈




书目名称Kidney Development读者反馈学科排名




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In Vitro Induction of Nephrogenesis in Mouse Metanephric Mesenchyme with Lithium Introduction and Urd molecular biology of the developmental process. The advantages of the tissue culture method include enhanced representativeness of situation in living organism compared to cell culture assays and less demanding and time-consuming possibilities to experimental work compared with in vivo research.
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Estimating Nephron Number in the Developing Kidney Using the Physical Disector/Fractionator Combinate accurate and precise estimates, unambiguous identification of developing nephrons is essential. Here, we describe a combined histochemical/stereological technique for estimating total nephron number in the developing mouse and rat kidney. The method can be modified for use in other species.
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Live Imaging of the Developing Mouse Mesonephross challenging in terms of embryo survival and minimization of phototoxicity. Here, we describe a procedure to image the developing mesonephros for up to 16 h in intact mouse embryos. This method can easily be adapted to the imaging of other structures at similar developmental stages.
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