Titlebook: Kidney Development; Methods and Protocol Odyssé Michos Book 2012 Springer Science+Business Media, LLC 2012 branching morphogenesis.cell mig

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ablate

Analysis of Native Kidney Structures in Three Dimensionselopment in rodents. Unlike confocal microscopy systems, OPT is capable of imaging the organ in toto across a long window of embryonic development at sufficient resolution to capture relative changes in branching dynamics, pelvis development, and nephrogenesis. Here, we describe how to image kidneys

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Estimating Nephron Number in the Developing Kidney Using the Physical Disector/Fractionator Combinat. However, until recently, a design-based method for estimating nephron number in the developing kidney was not available. For such a method to provide accurate and precise estimates, unambiguous identification of developing nephrons is essential. Here, we describe a combined histochemical/stereolog

小口啜饮

An Immunofluorescence Method to Analyze the Proliferation Status of Individual Nephron Segments in t, where up to 26 different cell types with highly specialized functions are present. Moreover, even though the nephron initially develops from a common progenitor pool, the individual nephron segments are ultimately quite different in respect to cell numbers. This suggests that some cells in the nep

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包租车船

Investigating Primary Cilia in Cultured Metanephric Mesenchymal Cellsembryonic kidney consists of two major cell lineages, ureteric and metanephric mesenchyme. Here, we describe a method to isolate metanephric mesenchyme from ureteric bud, culture metanephric mesenchyme cells, and study primary cilia in cell culture.

humectant

中国纪念碑

Engineered Tissues to Quantify Collective Cell Migration During Morphogenesistion of the ureteric tree, which develops from the repeated branching of the ureteric bud. During branching of the ureteric bud, cells migrate collectively in unison to form the complex structure of the tree. Here, we present a microlithography-based 3D culture model in which multiple identical kidn