Titlebook: Imaging Living Cells; Rosario Rizzuto,Cristina Fasolato Book 1999 Springer-Verlag Berlin Heidelberg 1999 Calcium.Calcium Dyes.Confocal Mic

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Strategies for Quantitative Digital Imaging of Biological Activity in Living Cells with Ion-Sensitivolled instrumentation for acquiring data from living tissue at fast rates has in turn made it possible to acquire ultra low light level data from these probes and to analyse either images or temporal changes in light emission, or both.

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High Resolution 3-D Imaging of Living Cells by Image Restorationoach integrates sensitive CCD cameras, wide field epifluorescence microscopes and a computational method, image restoration, to obtain 3-D images with a lateral resolution of 100 nanometers, which is better than the resolution that can be achieved by either confocal fluorescence microscopy or conven

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Imaging Calcium in the Cytoplasm and in Organelles with Fluorescent Dyes: General Principles ions were essential for the normal contraction of the frog heart. In 1957 Hodgkin and Keynes used .Ca. to trace the movements of the ion in the squid giant axon, and since that time many investigators have employed isotopes to examine Ca. fluxes in cells, tissues, or whole organs. These studies pro

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Confocal Calcium Imagings of the tissue above and below the plane of focus is rejected. Many of us who study intracellular calcium concentration ([Ca.].) use monolayers of cells in culture. After loading a calcium indicator dye into the cells, we place the cells on a fluorescence microscope and measure the signal from each

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