Titlebook: Imaging Living Cells; Rosario Rizzuto,Cristina Fasolato Book 1999 Springer-Verlag Berlin Heidelberg 1999 Calcium.Calcium Dyes.Confocal Mic

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Quantitative Calcium Imaging in Brain Slicesand enables to study visually-identified cell types. Since the majority of cells in a brain tissue slice preserve their structure and their synaptic contacts, this preparation is particularly useful to elucidate the properties of the synaptic interaction. Using Ca. imaging techniques, many intriguin

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Imaging of Cytosolic Sodium and pH Using SBFI and BCECF, tight regulation of other key ions is vital to the survival and normal function of cells. In particular, a considerable amount of their energy (20–70%) is expended via the plasma membrane Na./K.ATPase to ensure that the cytosolic Na. concentration ([Na.].) is relatively low compared with that in t

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Common Methods for Measuring Apoptotic Cell Death by Flow Cytometryn and in the control of immune response.. In some cases, it consists in an ordered sequence of cellular events that start with the transcription of specific genomic sequences, synthesis of specific proteins, and activation of nucleases that cut DNA in the oligonucleosomal subunits. In other models t

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Targeting, Expressing and Calibrating Recombinant Aequorinbeen responsible for the large expansion in the use of protein probes in cell biology. Two groups of reporter proteins are currently employed, derived from the wide variety of bioluminescent organisms: the chemiluminescent proteins (e.g. the different types of luciferases), which are endowed with an

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Imaging Ca2+ in Small Mammalian Cells with Recombinant Targeted Aequoriniving cells. Chief amongst these are: (i) zero toxicity; (ii) near-zero buffering of Ca.; (ii) low mobility; (iv) enormous dynamic range (0.1–100 µM); (v) ability to express the protein as a recombinant chimaera, and thus achieve targeting to subcellular domains where changes in [Ca.] would otherwis

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The New Green Fluorescent Protein Mutants and their Applicationsology. Briefly, GFP has been successfully used (i) as a fluorescent gene reporter., which offers several advantages over the chemiluminescent enzyme luciferase, (ii) as a morphological probe of different intracellular compartments,. and (iii) as a marker allowing the detection in intact cells of sev

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