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Titlebook: Imaging Living Cells; Rosario Rizzuto,Cristina Fasolato Book 1999 Springer-Verlag Berlin Heidelberg 1999 Calcium.Calcium Dyes.Confocal Mic

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书目名称Imaging Living Cells
编辑Rosario Rizzuto,Cristina Fasolato
视频video
概述First manual describing novel labelling strategies * Easy to follow step-by-step instructions * Practical hints and experimental strategies are given for every protocol
丛书名称Springer Lab Manuals
图书封面Titlebook: Imaging Living Cells;  Rosario Rizzuto,Cristina Fasolato Book 1999 Springer-Verlag Berlin Heidelberg 1999 Calcium.Calcium Dyes.Confocal Mic
描述Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Novel labelling strategies, better software for image enhancement and analysis are now available and allow image acquisition with greater speed and precision. This lab manual, intended as for bench-top use, is suitable for both scientists and graduate students, combines an update on the most advanced imaging procedures with detailed protocols. Examples, cleverly selected from the wide repertoire of cell pyhsiology, cover different functional aspects such as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.
出版日期Book 1999
关键词Calcium; Calcium Dyes; Confocal Microscopy; GFP; Path-Clamp Imaging; cells; cytoplasm; gene expression; prot
版次1
doihttps://doi.org/10.1007/978-3-642-60003-6
isbn_ebook978-3-642-60003-6
copyrightSpringer-Verlag Berlin Heidelberg 1999
The information of publication is updating

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are given for every protocolImproved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together
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Confocal Calcium Imaging of a number of individual cells. Under these circumstances, the only significant fluorescence is from the cell monolayer, and using a confocal microscope gives no significant improvement in performance.
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Targeting, Expressing and Calibrating Recombinant Aequorin excellent signal to noise ratio, and are widely employed, for example, for the monitoring of gene expression in transfected cells or organisms, and the fluorescent proteins. Among the latter, green fluorescent protein (GFP) of ., which has recently attracted a huge interest, will be discussed in other sections of this book.
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Imaging Green Fluorescent Proteins in Mammalian Cellsiments, with many different applications that for reason of brevity we will not be able to discuss. In this chapter, we will essentially describe how to look at Green Fluorescent Protein (GFP) and optimize its fluorescence signal.
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