Titlebook: Hematopoietic Stem Cell Protocols; Kevin D. Bunting,Cheng-Kui Qu Book 2014Latest edition Springer Science+Business Media New York 2014 gen

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Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Geneticallewal, long-term expansion, and in vitro myeloid or lymphoid transformation can be evaluated in human CD34. cells of fetal or adult origin that are engineered to express oncogenes. Together, these tools should allow a further molecular elucidation of derailed signal transduction in LSCs.

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Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Deve supportive cell types have been identified to play key roles in modulating adult HSC quiescence, proliferation, differentiation, and reconstitution. However, unlike in the adult bone marrow (BM), the components of stem cell niches, as well as their interactions with fetal HSC during different stage

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Single-Cell PCR Profiling of Gene Expression in Hematopoiesiss, including cancer, to infer routes of cellular specification and their respective gene regulatory modules. PCR-based technologies, although limited to the analysis of a predefined set of genes, afford a cost-effective balance of throughput and biological information and have become a method of cho

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Hematopoietic Stem and Progenitor Cell Mobilization in Mice, from umbilical cord blood or placental tissue, or from the peripheral blood after treatment of the donor with agents that enhance egress of HSPC into the circulation, a process known as “mobilization.” Mobilized peripheral blood stem cells (PBSC) have become the predominate hematopoietic graft for

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Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cellss of the bone marrow (BM) microenvironment known as the hematopoietic niche (HN). HSC enter into active phases of cell cycle in response to intrinsic and extrinsic biological cues thereby undergoing differentiation or self-renewal divisions. Quiescent and mitotically active HSC have different metabo

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Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)ecular signals mediating proliferation, mobilization, migration, and differentiation is believed to unravel disease-specific disturbances in hematological diseases and assist in the development of novel cell-based clinical therapies in this era of genomic medicine. The recent advent in genomic tools

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Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cellse decisions, mature immune effector cell function, apoptosis, and cell cycling, and a more limited number of miRNAs has been shown to regulate hematopoietic stem cell (HSC) self-renewal. Many of these miRNAs were initially identified as candidate regulators of HSC function by comparing miRNA express