Titlebook: Hematopoietic Stem Cell Protocols; Kevin D. Bunting,Cheng-Kui Qu Book 2014Latest edition Springer Science+Business Media New York 2014 gen

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DNA Methylation Profiling of Hematopoietic Stem CellsDNA methylation marks exist in the genome is crucial to understanding the role that methylation plays in hematopoiesis. This chapter describes a method to profile DNA methylation by selectively enriching methylated DNA sequences that are bound in vitro by methyl-binding domain (MBD) proteins. The MB

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Nanoproteomic Assays on Hematopoietic Stem Cellsing the biology of the pathways and the proteins involved. Studying these pathways in patient samples is challenging as samples are difficult to obtain, contain fewer cells, and are heterogeneous in nature. To address some of these difficulties, we have utilized the technique of microcapillary elect

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Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cellsf an organism. Here, we describe methods to isolate and culture murine bone marrow (BM) CD34.ckit.Sca1.Lineage. (CD34.KSL) hematopoietic stem cells (HSCs). We also describe a method to measure functional HSC content via the competitive repopulation assay. Furthermore, we summarize methods to isolate

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ations of advanced orientation field estimation techniques such as the method of fingerprint core extraction based on Poincaré indexes and model-based smoothing for the gradient-based approximation of an orientation field by Legendre polynomials, and new adaptive Gabor filtering technique based on h

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