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派博传思国际中心 »期刊书目

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Titlebook: Hematopoietic Stem Cell Protocols; Kevin D. Bunting,Cheng-Kui Qu Book 2014Latest edition Springer Science+Business Media New York 2014 gen

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书目名称Hematopoietic Stem Cell Protocols
编辑Kevin D. Bunting,Cheng-Kui Qu
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Hematopoietic Stem Cell Protocols; Kevin D. Bunting,Cheng-Kui Qu Book 2014Latest edition Springer Science+Business Media New York 2014 gen
描述.Updating and building upon previous editions, .Hematopoietic Stem Cell Protocols, Third Edition. provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases. An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Essential for the laboratory-based researcher, .Hematopoietic Stem Cell Protocols, Third Edition. is a much needed technical resource in the crit
出版日期Book 2014Latest edition
关键词gene expression; gene profiling; hematopoietic stem cells; heterogeneity; in vitro assays
版次3
doihttps://doi.org/10.1007/978-1-4939-1133-2
isbn_softcover978-1-4939-4757-7
isbn_ebook978-1-4939-1133-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
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Amber Hogart Begtrup% accuracy on the new dataset). We also tested several widely-used machine learning methods (logistic regression, Bernoulli Naïve Bayes) trained on the new question representation. The best result of 72.38% accuracy (micro) was achieved with the CNN model. We also ran experiments on pertinent featur
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% accuracy on the new dataset). We also tested several widely-used machine learning methods (logistic regression, Bernoulli Naïve Bayes) trained on the new question representation. The best result of 72.38% accuracy (micro) was achieved with the CNN model. We also ran experiments on pertinent featur
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Phuong L. Doan,John P. Chuteapproaches to detecting dialect features, and identified the advantages and disadvantages of each. Ultimately, we developed a unified algorithm that incorporates the best of the approaches considered, it takes a .wav file as input and returns .TextGrid and .eaf files with annotations and detected di
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Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Deveents. Herein, we describe methodologies for harvesting cells as well as the purification of stem and progenitors from fetal and newborn liver, spleen, and BM at various developmental stages following the expansion of hematopoiesis in the fetal liver at E14.5.
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Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cellsw. Because of the intracellular detection required for the HSC signaling assay, this assay also includes cell fixation and permeabilization. Gating strategies for assessing MFI and the frequency of Annexin V. apoptotic cells in a complex population are also described along with representative exampl
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