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Titlebook: Handbook for Rhizobia; Methods in Legume-Rh Padma Somasegaran,Heinz J. Hoben Book 1994 Springer-Verlag New York, Inc. 1994 bacteria.genetic

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Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobiacurring mutants that are resistant to high concentrations of certain antibiotics. This resistance may be used for recognizing rhizobial strains. In this chapter, two strains of . are used to produce spontaneous mutants resistant to streptomycin and spectinomycin. . mutants, with simultaneous resista
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enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification
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https://doi.org/10.1057/9780230596726amined. Simple stains of nodule smears will be examined under the microscope. Rhizobia will be isolated from nodules and grown on presumptive test media. The isolates will be authenticated on their original host plants and then preserved on ceramic beads.
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https://doi.org/10.1007/b112942termining the identities of the strains occupying the nodules. The soluble somatic antigen characteristics of a particular strain and its bacteroid form are demonstrated as useful markers in identifying strains in competition experiments.
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