Titlebook: Handbook for Rhizobia; Methods in Legume-Rh Padma Somasegaran,Heinz J. Hoben Book 1994 Springer-Verlag New York, Inc. 1994 bacteria.genetic

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Developing Antiseraobia. Antigens are prepared and then injected into rabbits by three different routes. Antisera are developed for serological techniques that require soluble and insoluble somatic antigens. Injection and bleeding techniques are practiced.

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Somatic Agglutination Reactions with Pure Cultures of Rhizobiaizobial antigen is heat treated to inactivate the flagellar antigens and to illustrate the granular clumps characteristic of the insoluble somatic antigen reaction. The highest dilution of the serum at which positive agglutination occurs is used to define the titer.

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Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusionins. In this chapter, soluble and diffusible heat-stable somatic antigens of varying molecular sizes are studied. The antigen and test antiserum are filled in separate wells and allowed to diffuse toward each other slowly through agar, which provides the support medium. Line(s) of precipitation woul

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Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusiontion for nodulation on soybean (.). The immunodiffusion technique, which is commonly employed for the detection of soluble antigens, is adopted for determining the identities of the strains occupying the nodules. The soluble somatic antigen characteristics of a particular strain and its bacteroid fo

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