Titlebook: Handbook for Rhizobia; Methods in Legume-Rh Padma Somasegaran,Heinz J. Hoben Book 1994 Springer-Verlag New York, Inc. 1994 bacteria.genetic

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Fabrizio D’Errico,Maurizio Dalla Casadetect and identify microorganisms simultaneously. FAs are useful for rhizobial strain identification in ecological research. In this chapter, antisera are purified by ammonium sulfate precipitations and dialysis. The protein content of the dialysate is determined. The immunoglobulin fraction is the

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https://doi.org/10.1007/978-3-319-22711-5bent assay (ELISA), the method described here can be used with a commercially prepared secondary anti-body-enzyme conjugate. In principle, the ELISA and the immunoblot assay are similar. In the immunoblot assay, the antigens are immobilized on a nitrocellulose membrane and reacted with strain-specif

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Collecting Nodules and Isolating Rhizobiaolates. The subfamilies in the . will be discussed and identifications will be made with the help of a botanical key. Nodules will be sectioned and examined. Simple stains of nodule smears will be examined under the microscope. Rhizobia will be isolated from nodules and grown on presumptive test med

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