Titlebook: Germinal Centers; Methods and Protocol Dinis Pedro Calado Book 2017 Springer Science+Business Media LLC 2017 GCs.Antibody immune responses.

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Follicular Dendritic Cell Isolation and Loading of Immune Complexes,s the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.

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Actuator Position Control Basics,systems, which recapitulate germinal centers, the most suitable way to study plasma cell generation in germinal centers is in vivo. In this chapter we describe how to induce humoral immune responses to defined model antigens and how to visualize and track plasma cells and their interactions with oth

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,Android® Programming with OLLOBOT SDK,thin and into/out of GCs plays a key role in the evolutionary process that underlies affinity maturation, and thus microanatomical location of cells is an important variable when studying GC processes. We describe a protocol in which in situ photoactivation by multiphoton microscopy can be used to a

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