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Titlebook: Germinal Centers; Methods and Protocol Dinis Pedro Calado Book 2017 Springer Science+Business Media LLC 2017 GCs.Antibody immune responses.

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书目名称Germinal Centers
副标题Methods and Protocol
编辑Dinis Pedro Calado
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Germinal Centers; Methods and Protocol Dinis Pedro Calado Book 2017 Springer Science+Business Media LLC 2017 GCs.Antibody immune responses.
描述.This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical, .Germinal Centers: Methods and Protocols .aims to ensure successful results in the further study of this vital field..
出版日期Book 2017
关键词GCs; Antibody immune responses; Vaccination; Cell-to-cell interaction; Cell activation
版次1
doihttps://doi.org/10.1007/978-1-4939-7095-7
isbn_softcover978-1-4939-8383-4
isbn_ebook978-1-4939-7095-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-Photon Photoconversion,es such as fluorescence-activated cell sorting and histology have provided numerous insights into the composition and function of the GC. However, these approaches are limited to a “snapshot” in time and are unable to fully capture the dynamic nature of the GC. Intravital two-photon microscopy overc
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,Intravital Microscopy of T–B Cell Interactions in Germinal Centers,ng affinity maturation and provided a wealth of information about how follicular helper T cells function in vivo. Here we describe a routine method to visualize fluorescence protein-expressing, antigen-specific T and B cells in germinal centers. The protocol for incorporating functional reporters or
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Identifying Follicular Regulatory T Cells by Confocal Microscopy,late the germinal center response. The anatomical positioning of follicular regulatory T cells within the germinal center is a defining characteristic of this subset of regulatory T cells; because of this, it is critical that studies of follicular regulatory T cells are able to identify them in situ
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