Titlebook: Genomic Imprinting; Methods and Protocol Andrew Ward Book 2002 Humana Press 2002

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Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting, division in the undifferentiated state by virtue of the action of exogenous factors. Leukemia inhibitory factor, LIF, is one such factor (2,3) and is indispensable for the propagation of mouse ES cells at least when primary embryo fibroblasts (PEFs) are used as feeder layers (4).

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Ribonuclease Protection,erse transcription step is not required. Finally, the RPA is quantitative as long as the probe is in excess. More important for the study of imprinted genes, the RPA can be designed to detect allele-specific expression of the target gene of interest.

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Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression, flank the sequence polymorphism, a method shown previously to yield amplicons whose allelic ratio is proportional to the ratio in the starting material, regardless of the number of cycles of amplification (.).

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Book 2002herited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1

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1064-3745 y, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation ex

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