Titlebook: Genomic Imprinting; Methods and Protocol Andrew Ward Book 2002 Humana Press 2002

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Allele-Specific , Hybridization (ASISH), list of genes that are expressed monoallelically fall into three main categories: random inactivation, allelic exclusion, and genomic imprinting. These categories are distinguished by whether the expressed and inactivated alleles are maintained from one cell division to the next (random inactivatio

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A PCR-Based Method for Studying DNA Methylation,occurs exclusively at CpG dinucleotides. CpG dinucleotides in the vertebrate genome are underrepresented and amount to 1% of the genome (.). However, in some regions of the genome, CpG residues amount to 6% or more of the dinucleotides in the genome. These regions, known as CpG islands, are usually

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Bisulfite-Based Methylation Analysis of Imprinted Genes,approximately 40 imprinted genes have been described that are exclusively or predominantly expressed from either the paternal or the maternal allele (www.mgu.har.mrc.ac.uk/imprinting/implink.html). Changes in the imprinted expression of such genes result in developmental abnormalities; in the human

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Safety Considerations of Pharmacotherapy, division in the undifferentiated state by virtue of the action of exogenous factors. Leukemia inhibitory factor, LIF, is one such factor (2,3) and is indispensable for the propagation of mouse ES cells at least when primary embryo fibroblasts (PEFs) are used as feeder layers (4).