Titlebook: Genomic Imprinting; Methods and Protocol Nora Engel Book 2012 Springer Science+Business Media, LLC 2012 Bioinformatics.Epigenetic profiles.

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Allison J. Hahr M.D.,Mark E. Molitch M.D.mic sperm injection, and round spermatid injection. By generating uniparental embryos that have exclusively paternal or maternal genomes, it is possible to study the functions of the parental genomes separately. It is possible to compare functions in haploid and diploid states. In addition, nuclear

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Diabetes and Protein Glycosylationo generate pluripotent stem cells directly from somatic cells, and these cells were named induced pluripotent stem cells (iPSCs). This method consists of technically simple procedures: donor cell preparation, gene transduction, and isolation of embryonic stem cell-like colonies. The iPSC technology

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Sami Oikarinen M.D.,Maarit Oikarinenish and maintain lineage-specific, monoallelic patterns of gene expression. This chapter describes the isolation an culture of trophectoderm stem cells from mouse blastocyst stage embryos. Using this powerful in vitro system, scientists can now begin to tease apart the epigenetic processes that resu

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Diabetes and the Endocrine Pancreasor the immunopurification of PGCs from the mouse embryo. After dissection and disruption of the fetal gonad, PGCs are identified by a monoclonal antibody recognizing an epitope characteristic of pluripotent stem cells. After reaction with a paramagnetic bead-linked secondary antibody, the cell mixtu

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Diabetes bei Kindern und Jugendlichenant approach for mapping allelic imbalance (AI), where transcription is biased toward one allele in a diploid system. AI is identified by counting sequencing reads that map to genomic regions containing heterozygous SNPs, where the base identity of the SNP is used to distinguish allelic origin. Geno

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Thomas Danne,Olga Kordonouri,Karin Langeeralized linear models (glm) to predicted imprinted status. This is meant to be an introduction to the method, so only the most straightforward examples will be covered. For instance, the examples given below refer to 11 classes of genomic regions (the entire gene body, introns, exons, 5′ UTR, 3′ UT