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Titlebook: Genomic Imprinting; Methods and Protocol Nora Engel Book 2012 Springer Science+Business Media, LLC 2012 Bioinformatics.Epigenetic profiles.

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书目名称Genomic Imprinting
副标题Methods and Protocol
编辑Nora Engel
视频video
概述Surveys cutting-edge technologies applied in the field.Features detailed and practical laboratory protocols.Includes expert tips on troubleshooting and avoiding pitfalls.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Genomic Imprinting; Methods and Protocol Nora Engel Book 2012 Springer Science+Business Media, LLC 2012 Bioinformatics.Epigenetic profiles.
描述.Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In .Genomic Imprinting: Methods and Protocols., experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting.  This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers.  Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Authorita
出版日期Book 2012
关键词Bioinformatics; Epigenetic profiles; Gene regulation; Genomic evolution; Imprinted genes; Parent-of-origi
版次1
doihttps://doi.org/10.1007/978-1-62703-011-3
isbn_softcover978-1-4939-5966-2
isbn_ebook978-1-62703-011-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2012
The information of publication is updating

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Data Mining as a Discovery Tool for Imprinted Geneseralized linear models (glm) to predicted imprinted status. This is meant to be an introduction to the method, so only the most straightforward examples will be covered. For instance, the examples given below refer to 11 classes of genomic regions (the entire gene body, introns, exons, 5′ UTR, 3′ UT
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Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cellse is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody that specifically recognizes 5-methylcytidine (5mC), which can be combined with PCR or high-throughput analysis (microarrays, deep sequencing). The MeDIP technique has been originally used to generate DNA
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