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Titlebook: Gene Therapy Protocols; Jeffrey R. Morgan Book 20022nd edition Humana Press 2002

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楼主: endocarditis
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Bioartificial Muscles in Gene Therapy,by means of retroviral transduction or stable transfection has produced cells secreting a vast variety of growth factors, including insulin (.), granulocyte colony-stimulating factor (.), growth hormone (.,.), dopamine (.), and erythropoietin (.). These cells can then be transplanted back into the body to provide a therapeutic protein treatment.
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Preparation of Pseudotyped Retroviral Vector,easing viral titers by ultracentrifugation (.); they were able to produce an average transduction efficiency of 10–60%. However, all such improvements in transduction efficiency require additional procedures, which are practically inefficient.
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https://doi.org/10.1007/978-1-349-22719-8erol LPD is 1200 nmol DOTAP/1200 nmol cholesterol/60 μg protamine sulfate/100 μ plasmid DNA, which has a charge ratio of 4:1 (+:-) between DOTAP and DNA and a 1/1 charge ratio between protamine and DNA. The optimal composition for DC-Chol/DOPE LPD is 60 nmol total lipids (36 nmol DC-Chol and 24 nmol DOPE)/80 μg protamine/100 μ plasmid DNA.
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Mauro Cappelletti,Joseph M. Perillor dermis is relatively nonimmunogenic and retains many of its structural elements after processing. Skin substitutes using acellular dermis can be formed in vitro and subsequently transplanted to athymic mice, generating a well-differentiated and fully pigmented epidermis with many characteristics of normal skin (.,.).
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Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery,peatedly, and be modified with appropriate ligands that allow specific cell targeting. Until now, many different types of organic compounds have been tested as synthetic gene delivery vectors in animal models by various routes of administration (. . .–.).
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