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Titlebook: Gene Therapy Protocols; Jeffrey R. Morgan Book 20022nd edition Humana Press 2002

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发表于 2025-3-21 18:48:05 | 显示全部楼层 |阅读模式
书目名称Gene Therapy Protocols
编辑Jeffrey R. Morgan
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Gene Therapy Protocols;  Jeffrey R. Morgan Book 20022nd edition Humana Press 2002
描述Efforts in gene therapy have grown dramatically in recent years. Basic research as well as clinical activity have made exciting progress and are beg- ning to offer renewed hope that gene therapy may be able to deliver novel approaches for the treatment of inherited as well as such acquired diseases as cardiovascular disease and cancer. With the sequencing of the human genome complete, we now have a comprehensive catalog of genes that further expands the potential role of gene therapy into such new fields as tissue engineering. Central to gene therapy is the process of gene transfer; thus, advances in the technology of gene transfer are at the heart of this field’s progress. Numerous technologies, based on a variety of methods (e.g., viral-mediated, physical/ chemical), have been developed to achieve gene transfer. Some of the earliest methods, such as recombinant retroviruses, are still widely used, have undergone significant improvements, and have given rise to new vectors based on lentiviruses.
出版日期Book 20022nd edition
版次2
doihttps://doi.org/10.1385/1592591418
isbn_ebook978-1-59259-141-1Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2002
The information of publication is updating

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https://doi.org/10.1007/978-1-349-03526-7peatedly, and be modified with appropriate ligands that allow specific cell targeting. Until now, many different types of organic compounds have been tested as synthetic gene delivery vectors in animal models by various routes of administration (. . .–.).
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https://doi.org/10.1007/978-90-6704-817-0easingly important. However, work from our laboratory as well as others has shown that the most commonly used quantitative retrovirus measures (i.e., titer and multiplicity of infection [MOI]) cannot be used to make accurate comparisons (.–.).
发表于 2025-3-22 10:37:53 | 显示全部楼层
https://doi.org/10.1057/9780230621749 goal is a single gene transfer event that would lead to life-long correction of the genetic defect. Moreover, retroviral vectors are thoroughly characterized, and their extensive use in preclinical and clinical studies has verified their efficacy and safety.
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https://doi.org/10.1007/978-981-99-1300-8d that gene transfer into muscle by electroporation in vivo is far more efficient than simple intramuscular DNA injection and provides a potential approach toward systemic delivery of cytokines, growth factors, and other serum proteins for human gene therapy.
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Site surveys, investigations and layout,tissues and was later extended to various ex vivo gene transfer systems, including excised tissue explants or clumps, organic tissues placed in culture vessels, and their derived primary cultures (.; . .).
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