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Titlebook: Gene Targeting Protocols; Eric B. Kmiec Book 2000 Springer Science+Business Media New York 2000

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Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vecto-mediated endocytosis, resulting in selective killing of the target cells. However, the low level of Fab(105)-protamine fusion protein secreted from the stably transduced COS cells has been a limiting factor for experiments requiring large amounts of the fusion protein.
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Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylaterent strategies for recognizing DNA can be directly compared. The ability to detect and quantify an early, perhaps rate limiting step, in genetic targeting should be useful in efforts to better understand and improve this important technique.
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Eric B. KmiecIncludes supplementary material:
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978-1-4899-4158-9Springer Science+Business Media New York 2000
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Gene Targeting Protocols978-1-59259-215-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Kumiko Ito-Miwa,Kazuki Terauchi,Takao KondoAntisense oligonucleotides have been extensively used as downregulators of gene expression (.), and as such are not only increasingly used in various fields as sequence-specific research tools (.–.), but are also tested in clinical trials as antiviral and anticancer agents (.–.).
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https://doi.org/10.1007/978-3-030-70590-9n recent years. The principal reasons for this have been the ease with which the methodology can be applied to a wide range of cell types; the relatively low cytotoxicity compared to other techniques; the high efficiency of nucleic acid transfer in comparison with methods such as calcium phosphate o
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