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Titlebook: Experimental Cholestasis Research; Mathieu Vinken Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Drug-induce

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发表于 2025-3-21 17:08:21 | 显示全部楼层 |阅读模式
书目名称Experimental Cholestasis Research
编辑Mathieu Vinken
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Experimental Cholestasis Research;  Mathieu Vinken Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Drug-induce
描述This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Experimental Cholestasis Research. serves as an ideal guide for basic andapplied researchers pursuing this vital area of research in pharmacology and toxicology..
出版日期Book 2019
关键词Drug-induced liver injury; Liver toxicology; DILI; Cholestatic drug properties; Model organisms; Impaired
版次1
doihttps://doi.org/10.1007/978-1-4939-9420-5
isbn_softcover978-1-4939-9422-9
isbn_ebook978-1-4939-9420-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Functionsubstrate signal can be determined in the cells and bile or the cells alone, respectively. Performing this assay in the presence and absence of potentially interfering compounds of interest enables exploration of the relative effect of these compounds on biliary excretion of the probe substrate.
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In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids,sporters in mice, using the nuclear imaging techniques positron emission tomography and single photon emission computed tomography. The protocol includes detailed information on preparation of the animal, scan acquisition, processing, and (statistical) analysis.
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Transcriptomic Analysis of Cholestatic Compounds In Vitro,r repeat exposure-related toxicities, as well as for the study of adaptive responses. This primary human hepatocyte culture system combined with transcriptomics carries the future promise to identify individual gene expression profiles predictive of increased drug-induced cholestasis risk..This chap
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System Microscopy of Stress Response Pathways in Cholestasis Research,having cholestasis liability and affected genes during cholestatic injury on cellular adaptive stress pathway activation. The approach involves high-throughput live-cell visualization of GFP-tagged key proteins of the oxidative stress response/Nrf2 pathway and inflammatory cytokine signaling. Quanti
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1064-3745 .Experimental Cholestasis Research. serves as an ideal guide for basic andapplied researchers pursuing this vital area of research in pharmacology and toxicology..978-1-4939-9422-9978-1-4939-9420-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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